SIRT2 Knockout cell line(A549)

ENCN
location： Home > Products > KO Cell Lines > SIRT2 Knockout cell line(A549)

SIRT2 Knockout cell line(A549) Order

SIRT2 Knockout cell line(A549)
Catalog#
YKO-H170
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SIRT2 Knockout cell line(A549)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SIRT2 Knockout cell line (A549)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
SIRT2
Gene ID
22933
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% F12K+10% FBS
Accession
CVCL_D8AA(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 4 (based on transcript SIRT2-201)
Validation: PCR+Sanger Sequencing
SIRT2 Knockout cell line(A549) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SIRT2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A549 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SIRT2 gene knockout SIRT2 Knockout cell line (A549) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SIRT2 Knockout cell line(A549)
SIRT2 Knockout cell line(A549)
Catalog#
YKO-H170
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • SIRT2 Knockout cell line (H9)

    SIRT2 Knockout cell line (H9)
      inquiry   More details

    Organism: Human
    Gene Name: SIRT2
    Gene ID: 22933
    Size: 1*10^6
    Catalog#: YKO-H1123
    YKO-H1123(H9)
  • SIRT2 Knockout cell line (HeLa)

    SIRT2 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: SIRT2
    Gene ID: 22933
    Size: 1*10^6
    Catalog#: YKO-HS4481
    YKO-HS4481(HeLa)
  • SIRT2 Knockout cell line (HCT 116)

    SIRT2 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: SIRT2
    Gene ID: 22933
    Size: 1*10^6
    Catalog#: YKO-HS3358
    YKO-HS3358(HCT 116)

Related Popular Products

Wild-type Cell Lines

  • A549 cell line

    Human Non-small Cell Lung Cancer Cell Line(A549)

    Price (USD): -
    Catalog#: YC-C016
    Size: 1*10^6
    Instruction: Human Non-small Cell Lung Cancer Cell Line(A549)
      inquiry   More details

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • SIRT2 Knockout cell line (H9)

    SIRT2 Knockout cell line (H9)

    Organism: Human
    Gene Name: SIRT2
    Gene ID: 22933
    Size: 1*10^6
    Catalog#: YKO-H1123
      inquiry   More details
  • SIRT2 Knockout cell line (HeLa)

    SIRT2 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: SIRT2
    Gene ID: 22933
    Size: 1*10^6
    Catalog#: YKO-HS4481
      inquiry   More details
  • SIRT2 Knockout cell line (HCT 116)

    SIRT2 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: SIRT2
    Gene ID: 22933
    Size: 1*10^6
    Catalog#: YKO-HS3358
      inquiry   More details
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now